Amplified Fragment Length Polymorphism
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In Situ PCR
(9)
RNA to DNA Amplification
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Real Time PCR
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Software
(8)
Thermostable DNA Polymerases
Discussion of their origin and briefly their properties. From the web site of Colorado State University.
RAPD PCR
RAPD stands for Random Amplification of Polymorphic DNA, where the target sequence(s) (to be amplified) is unknown.Brief description, from Rutgers University, USA.
Degenerate PCR
An introduction with principle, applications and description of the technique. From the web site of Kebangsaan University, Malaysia.
Anchor Probes for Comparative Mapping of Grass Species
Article in which probes from different libraries were used to hybridize seven cereals at the Department of Plant Breeding and Biometry, Cornell University, NY.
PCR method protocols
Protocols for PCR posted by the Helen Donis-Keller Laboratory.
Detection of Asymmetric PCR Products in Homogeneous Solution by Fluorescence Correlation Spectroscopy
In asymmetric PCR, the low concentration primer is quantitatively incorporated into double stranded DNA after an appropriate number of cycles. If this primer is fluorescent labelled, the dsDNA can be quantitated from the fluorescence.
FISH Guide and Troubleshooting
Links to pages describing influential parameters, with guides on PCR, RT-PCR and multiplex PCR reactions, Taq, FISH, CM-FISH, TM-FISH, microarrays, CCK, slide prep and labeling, maintained by Octavian Henegariu from Yale University, New Haven, CT.
Tavi's PCR protocols
A page describing the main parameters and trouble-shooting in PCR. The page is somewhat dated (updated 1997) but still useful.
PCR Troubleshooting
Limited to conventional straight forward PCR. Page designed and maintained by Octavian Henegariu on the web site of the Yale - New Haven Medical Center.
Adjuvants in PCR Reactions
Brief discussion of additives to improve amplification efficiency and specificity of PCR, by Octavian Henegariu, Yale-New Haven Medical Center.
C. elegans Gene Knockout Project
Protocols including standard nested PCR, from the Biotechnology Laboratory, University of British Columbia.
The "Poison Primer" Technique for Enhancing Detection of Small Deletions in Mutant Libraries
This method works because the poison allows the formation of deletion products but titers out full-sized products. From the Biotechnology Laboratory, University of British Columbia.
Rational primer design greatly improves differential display-PCR (DD-PCR)
Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.
Nematode ITS1 Size Variation
Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes.
Kary B. Mullis - Autobiography
The originator of PCR, from the Nobel e-museum web site.
RAPD's
Brief description of Random Amplified Polymorphic DNA, from Oklahoma State University, USA.
What the Heck is PCR?
Popular description of the PCR technique by John C Brown, University of Kansas 1995.
A Rapid DNA Minipreparation Method Suitable for AFLP and Other PCR Applications (PDF document)
Preparation of DNA from plant tissues suitable for PCR methods including AFLP, article by DH CHEN and PC RONALD Department of Plant Pathology, University of California, Davis.
Detection of Single Nucleotide Mutations in Wheat Using Single Strand Conformation Polymorphism Gels (PDF file)
P Martins-Lopez, H Zhang, R Koebner, Plant Mol. Biol. Reporter 19(2001): 159-162. From National Research Coouncil Canada.
Design Oligonunucleotide Primers
Interface for scanning DNA sequences for restriction sites, without changing encoded protein created. Includes FAQ, table of enzymes and links to tools, maintained at the University of Waterloo, ON, Canada.
PCR Project
Presentations from the University of California at Berkley on PCR, both current research reports and reviews.
Long PCR Protocol
Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA
Principle of PCR
Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium.
PCR animated
Animation illustrating the principle of PCR, from the University of Ghent, Belgium.
RFLP Definition
RFLP = Restriction Fragment Length Polymorphism, from FDA
Technical manual of PCR
Intended for specialists planning PCR procedures in their laboratories. From the University of Cape Town updated 2001.
Standard PCR protocols
From Molecular Biology Techniques Manual, from the web site of the University of Cape Town, South Africa.
PCR primer design and reaction optimisation
Article by Ed Rybicki, Department of Molecular and Cell Biology, University of Cape Town in: Molecular Biology Techniques Manual, on the web site of the University of Cape Town.
Which DNA Marker for Which Purpose?
Compendia of the Research Project "Development, optimisation and validation of molecular tools for assessment of biodiversity in forest trees", European Union DGXII Biotechnology FW IV Research Programme. From the web site of the University Library, Göttingen.
Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences
RFLP = Restriction Fragment Length Polymorphism. By Alexander Binder 1997.
Wayward PCR primers
Article by PN Hengen from TIBS 1995 on the loss of activity of PCR primers with time.
Optimizing Multiplex and LA-PCR with Betaine
LA-PCR = "long and accurate PCR". Article by PN Hengen in TIBS June 1997.
Single tube confirmation PCR protocol
For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA.
Polymerase Chain Reaction
Popular survey article by Mark V. Bloom, DNA Learning Center, Cold Spring Harbor Laboratory, from the web site of the US National Health Museum.
Polymerase Chain Reaction (PCR)
A graphic description of the principle of PCR from the US National Health Museum web site.
PCR Technology
An introduction by Connie Veilleux from the US National Health Museum website.
Attotron Biosensor Corporation
R & D company for development of biosensors and related products for the research and educational markets.
Life at High Temperatures
Article by TD Brock from the web site of Department of Bacteriology, University of Wisconsin - Madison on the thermophilic organisms in Yellowstone hot springs, including Thermus aquaticus from which Taq polymerase was obtained.
PCR Protocol
Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.
Inverse PCR protocol
step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.
Biosource molecular methods booklet
56 page booklet on oligonucleotides, PCR and RT-PCR, from Biosource (company).
Improved diagnosis of porcine proliferative enteropathy caused by Lawsonia intracellularis using polymerase chain reaction-enzyme-linked oligosorbent assay
P Zhang, CJ Gebhart, D Burden, GE Duhamel: Molecular and Cellular Probes, 14(2) 101-108, on the site of BT&C, Inc.
PCR Protocols
Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State University
Alu-PCR Hybridization
A report and protocol, from Fondation Jean Dausset.
Degenerate PCR
The identification of novel members of gene families by PCR using degenerate primers is described and protocols given. Article by Michael Koelle 1996 on the web site of Dartmouth College.
T-DNA Generated Enhancer Traps in Arabidopsis
Application of inverse PCR, partial genomic libraries and TAIL-PCR in cloning flanking, at the Department of Biological Sciences, Dartmouth College, Hanover, NH.
Dolan DNA Learning Centers Gene Almanac
Educational site on topics in genetics and gene expression from Cold Spring Harbor Laboratory, USA.
DNALC: PCR Animation
An animation explaining how the Polymerase Chain Reaction (PCR) works, from the Dolan DNA learning center, Cold Spring Harbor Laboratory.
Random Arbitrarily Primed PCR
Protocol for RAP-PCR to study prokaryotic gene expression, from the Enterococcus Research Site, University of Oklahoma, USA.
General Notes on Primer Design in PCR
Article on the influence of the design of the primer on the outcome of PCR, by Dr Vincent R. Prezioso, published by Eppendorf, North America.
Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
Basic discussion of the optimization of PCR, from the web site of Brinkmann Instruments.
The Polymerase Chain Reaction
A popular description of the way it works, its importance in different connections, legal aspects. Authored by TM Powledge at the Federation of the American Societies of Experimental Biology.
RAPD analysis with P. infestans
Protocol for Randomly Amplified Polymorphic DNA, by H Judelson, University of California, Riverside, USA.
Inverse PCR For use with Snyder mTn-lacZ/LEU2 Based Mutagenesis
Protocol by M McMurray, Fred Hutchinson Cancer Research Center, Seattle, Wa. USA.
Inverse PCR and Cycle Sequencing of P Element Insertions for STS Generation
Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.
Cleaved Amplified Polymorphic Sequence
Illustration of principle. CAPS corresponds to RFLP. From Corvallis Forestry Research Community, Corvallis, USA.
Reference in PCR
Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers.
GeneOhm Sciences
Tests on gruup B Streptococcus and methicillin resistant Staphylococcus aureus by PCR / DNA sequencing.
Inverse PCR for PAC-end sequencing
To generate PCR fragments that contain the ends of PAC inserts that can be sequenced. Protocol by B Barbazuk, Washington University Zebrafish Genome Resources Project, USA.
GenHunter
Manufacturer of material for differential display PCR in Nashville, Tenn USA.
Roe Laboratory Protocols
Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.
HiFi DNA
HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.
PCR and multiplex PCR guide
Discussions of the parameters influencing the PCR reaction and some PCR and multiplex PCR applications, by Octavian Henegariu on the web site of the Yale - New Haven Medical center.
Ingenetix GmbH
Develops technology and products for DNA and mRNA research. Also provide DNA testing for the determination of parentage/paternity and custom DNA sequencing, oligonucleotide synthesis, genotyping services, pharmacogenetics and quantitative PCR.
Kary Mullis
Inventor Profile of Kary Mullis, the originator of PCR, from the National Inventors Hall of Fame web site.
Quantitative PCR Protocol
From the Jackson Laboratory, University of Maine, USA.
Optimizing PCR Protocols
Brief guidelines. From the Jackson Laboratory, University of Maine, USA.
Fidelity of DNA Polymerases for PCR
Article by PN Hengen from TIBS 1995
MJ Research
Manufactures and sells products for molecular biology including thermal cyclers and sequencers / genotypers.
Rep-PCR Genomic Fingerprinting
Bacteria are characterized by Rep-PCR fingerprinting using primers corresponding to naturally occurring repetitive sequences in the interspersed regions.
PCR Amplification of cDNA Segments by 2 Stage Nested PCR
Protocol from the method database of NIH, USA.
RACE amplification
Rapid Amplification of cDNA Ends. Protocol, from National Institute of Genetics, Japan.
3' RACE PCR
This technique is used to obtain the 3'end of a cDNA. Protocol by R Hastings, Biochemistry, University of Nottingham Medical School, UK.
The web guide of PCR
List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina.
Protocols Online: PCR Protocols
Extensive collection of PCR protocols and methods from Protocol On Line.
Effect of PCR Buffer on multiplex PCR (PDF)
Multiplex PCR employs different primer pairs in the same amplification reaction. This requires extensive optimization of annealing conditions. From Quiagen (company)
Innovative Tools for Amplification
Notes about PCR applications, principles, research results, from Roche.
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