Comparative Genomic Hybridization
CGH is a molecular cytogenetic method of screening a tumor for genetic changes. CGH protocols, images, publications, events and links maintained by the University Hospital Charité, Humboldt-University of Berlin, Germany.
BioProtocol
Internet project initiated in early 1992 as BRNI, bio.com includes PCR, hybridization and microarray protocols.
Comet Assay Interest Group
Forum for exchange of views on the single cell gel electrophoresis system, capable of detecting single- and double-strand breaks, oxidative DNA base damage, and DNA, protein and drug crosslinking. Includes related links.
ECR Browser
Multiple alignment and dynamic evolutionary conservation profile of several vertebrate genomes including human, mouse, rat, chicken, frog, and three fishes, developed at LLNL, Livermore, California.
SNP Scoring
Overview of genotyping, detection and quantifying single nucleotide polymorphism, including the application of template directed dye terminator incorporation assay. Offers database and links to other resources from PerkinElmer Life Sciences, Boston, MA.
Molecular Biology Protocols
Protocol collection, with interactive forum for over ten topics, covering DNA, PCR, RNA and proteins techniques and links to online journals and bioinformatics resources, from the Northwest Fisheries Science Center Microbial Pathogenesis Lab, Seattle, WA.
Practical Molecular Biology
Methods, information, programs and BioMail for molecular biologists, as joint venture between Germany and Russia.
SciWeb Protocols and Reagents
Directory of molecular biology protocol links.
Surgery Research
Collection of DNA microarray technology protocols, primarily for resident students at Stanford.
Home Page of Andy Vierstraete
The use of DNA techniques in phylogenesis. Explanations, protocols and animations from Andy Vierstraete at the University of Ghent, Belgium.
Recombinant DNA
Preparing probe, cloning into plasmids, complementarity and hybridization, gel electrophoresis, transfer to solid support and blotting variants, blocking, target detection PCR problems from chapter in MIT hypertext handbook.
Assay of Protein Concentration
Different methods of determining protein concentration, as a resource for researchers and students in biochemistry and molecular biology laboratories from University of Nebraska, Lincoln, NE.
Journal of Biochemical and Biophysical Methods
Publishes research papers dealing with the development of new methods or the significant modification of existing techniques to solve biological problems.
Analytical Biochemistry
Emphasizes methods in the biological and biochemical sciences.
Reto's Buffers and Media Book
Alphabetical list for buffer recipes, including constituents for cultures, from a personal page in Switzerland.
MolBio-Spot
Richard Man-kit Yu's site where you can acquire various molecular biology techniques.
Basic Principles of Gene Cloning, Sequencing, & Analysis
Description of methods behind gene cloning, genetic engineering, DNA recombination and isolation, including discussion of restriction enzymes and ligase.
Molecular Biology Protocols
Resources such as polymerase chain reaction, dedicated to method development. Part of internet directory maintained by Horizon Scientific Press, Wymondham, England.
Molbiol.net
Resource for molecular biologists on methods, with feedback for user-directed evolution and links to commercial suppliers from Jonathan D Rees, Oxford, UK.
Molecular Cloning Manual
Subscription only source of laboratory techniques in DNA and protein science, to complement the hard copy, giving scientists the benefit of both media from CSHL, NY.
Owczarzy, Richard
Online calculations of nucleic acid extinction coefficients, melting temperatures, and links to biophysics databases. Includes thesis, publications and contacts for commercial scientist in Coralville, Iowa.
Molecular Probes, Inc.
Handbook of fluorescence applications, bibliography, data tables and calculators for molecular biology, information on courses and workshops from supplier in Eugene, OR and Leiden, Netherlands.
Beginning Laboratory Manual
Teaching manual to support the applied biotechnology programs at the University of Maryland, Baltimore.
Tronolab Website
Collection of protocols, maps and sequences regarding manipulation of lentiviral vectors, siRNA and transgenic animals. Applications in HIV, stem cell and gene therapy research at University of Geneva.
BBHelp
Collection of molecular biology techniques derived from help files of Dr. Harold Zappe at the Department of Medical Microbiology, University of Cape Town, South Africa.
Protocols
Microsatellites and others techniques from the Savana River Ecology Laboratory, University of Georgia.
Hirsh Lab Gel Documentation System
Description of an agarose gel set-up. Includes instructions for camera operation with a script, with computer side image analysis from the University of Virginia, Charlottesville.
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